The aim of this paper is to present the design and implementation of a teaching intervention on differentiated instruction that was developed for the 4th Grade of primary school, alongside the cooperation of various teachers. The novelty of this teaching plan is that an experimental interdisciplinary approach is attempted in differentiated instruction, in order to bring together three different school subjects (Mathematics, English, Physical Education). In particular, this complex didactic scenario is structured within theframework of differentiated instruction, focuses on one main topic (problem solving on four mathematical operations) and identifies individual goals and concepts related to the different school subjects involved. The theoretical approach, the methodology and the development phases of the lesson are presented,including examples of activities. Finally, there is an evaluation of the impactof this innovative approach on the extend of the effectiveness of learning.